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Do the B2 sub-class of cyclins mediate a cytokinin response in G2?

Reference	P13340
Principal Investigator / Supervisor	Professor Laszlo Bogre
Co-Investigators / Co-Supervisors	Professor John Doonan
Institution	Royal Holloway, Univ of London
Department	Biological Sciences
Funding type	Research
Value (£)	230,444
Status	Completed
Type	Research Grant
Start date	01/11/2000
End date	01/11/2003
Duration	36 months

Abstract

The ectopic expression of a cycB2 gene in tobacco shortens G2 phase and alters development, mimicking certain cytokinin responses, unlike over-expression of a cycB1 gene in Arabidopsis, which seems to speed up growth but does not affect development. To unravel role of B-type cyclins in cytokinin response we propose: 1) to up and down-regulate B2-cyclins and over-express a B1-cyclin in WT and mutant Arabidopsis lines with altered cytokinin content or sensitivity and compare their effect on cell cycle progression by flow cytometry and on cytokinin responses; and 2) to identify the CDK partner(s) for B1 and B2 cyclins, and thus to study the effect of cytokinin on the activity and tyrosine phosphorylation of specific CDK(s).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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