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Characterisation of the Reg signalling pathway in Rhodobacter sphaeroides
Reference
P13277
Principal Investigator / Supervisor
Dr Mary Phillips-Jones
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
152,396
Status
Completed
Type
Research Grant
Start date
02/10/2000
End date
02/10/2003
Duration
36 months
Abstract
The proposed study will identify new components and confirm/further characterise some suspected/known components of Reg, a signal transduction pathway that regulates and integrates the expression of genes involved in photosynthesis, reductive assimilation of CO2 and nitrogen fixation, in response to external changes in aerobiosis/anaerobiosis conditions. The main approach will be the characterisation of a series of recently isolated second-site suppressor mutants. Location of second-site mutations in regA and regB mutants will be identified using PCR- SSCP, AP-PCR, complementation, and 2D-protein gel analysis. Identified components of interest will be characterised by gene cloning, sequencing and inactivation. To determine whether these components are process specific, their effects on each of the biological processes described above will also be examined.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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