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Investigation of Cf-9 protein function by characterisation of Cf-9myc interacting proteins before and after elicitation
Reference
P13272
Principal Investigator / Supervisor
Professor Jonathan Jones
Co-Investigators /
Co-Supervisors
Institution
University of East Anglia
Department
Sainsbury Laboratory
Funding type
Research
Value (£)
347,712
Status
Completed
Type
Research Grant
Start date
01/05/2000
End date
31/12/2004
Duration
56 months
Abstract
The tomato Cf-9 gene confers resistance to races of Cladosporium fulvum that carry Avr9. In tobacco Cf-9 confers defence responses upon Avr9 elicitation. Insertion of a triple myc tag at the N-terminus or C-terminus of Cf-9 does not abolish Cf-9 function. Cf-9myc can be immunoprecipitated with antimyc antibodies. Upon gel filtration of solubilised microsomes, Cf- 9myc elutes as an approximately 600 kDa complex; we will test if radiolabelled Avr9 associates with this complex. Using coimmunoprecipitation, evidence has been obtained for phosphorylation of Cf-9myc-associated proteins on elicitation. We propose to isolate enough associated protein(s) for protein microsequencing and isolation of the corresponding genes. Elicitation-dependent changes in the identity and post-translational modification of associated proteins will be investigated.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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