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Molecular analysis of Cf-2/Cf-5 disease resistance gene recognition specificity and function
Reference
P13228
Principal Investigator / Supervisor
Dr Mark Dixon
Co-Investigators /
Co-Supervisors
Institution
University of Southampton
Department
Centre for Biological Sciences
Funding type
Research
Value (£)
166,144
Status
Completed
Type
Research Grant
Start date
01/08/2000
End date
30/09/2003
Duration
38 months
Abstract
The availability of two closely related cloned resistance genes encoding different specificities in the tomato/Cladosporium fulvum interaction provides an excellent opportunity to probe the function of these genes. Precise domain swaps between Cf-2 and Cf-5 will be introduced into transgenic plants and will establish regions involved in determining specificity and signalling. Crosses between these transgenic lines and a line that carries a mutation in a gene (Rcr3), absolutely required for Cf-2 but not Cf-5, will determine whether Rcr3 is required for signalling or avirulence recognition. Further analysis of Cf protein function will be carried out by conventional biochemical analysis through the insertion of an epitope tag into Cf-2.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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