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RNA structural elements and RNA-polymerase interactions in the replication of a mitochondrial RNA virus
Reference
P13218
Principal Investigator / Supervisor
Professor Kenneth Buck
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
171,056
Status
Completed
Type
Research Grant
Start date
01/10/2000
End date
01/10/2003
Duration
36 months
Abstract
The structure of the 3' and 5' untranslated regions of the RNA of a fungal mitochondrial RNA virus (mitovirus) will be determined with chemical and enzymatic probes. The roles of 5'- and 3'- terminal structural elements in mitovirus RNA replication will be investigated by in vitro mutagenesis using both in vitro RNA synthesis with a template-dependent RNA polymerase and in vivo RNA synthesis. Template competition, and gel mobility shift experiments will be used to investigate interactions of the mitovirus RNA polymerase with RNA structural elements. Essential cis-acting sequences will be used to construct replicating mitochondrial vectors which could have widespread application in mitochondrial biology.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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