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Pollen-pistil interactions and transduction mechanisms involving cytosolic calcium

Reference	P13216
Principal Investigator / Supervisor	Professor Tony Trewavas
Co-Investigators / Co-Supervisors
Institution	University of Edinburgh
Department	Inst of Cell and Molecular Biology
Funding type	Research
Value (£)	77,996
Status	Completed
Type	Research Grant
Start date	12/07/2000
End date	12/09/2002
Duration	26 months

Abstract

Pollen and pistil engage in a continuous signal conversation as pollen tubes grow towards the ovule. The significance of cytosolic calcium in translating this conversation will be investigated. Cytosolic calcium signals will be measured and imaged using transgenic aequorin in the pistil and in pollen during compatible and incongruous combinations and in pollen guidance mutants. Tobacco flowers with modified pistil calmodulin and CDPK contents will test the involvement of these two calcium dependent proteins in controlling pollen tube growth in the stigma, style and ovary. Finally the putative role of pistil-synthesised reactive oxygen species in slowing pollen tube growth in incongruous pistil situations will be investigated.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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