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Biochemical and genetic analyses of the Arabidopsis Toc complex
Reference
P12928
Principal Investigator / Supervisor
Professor Paul Jarvis
Co-Investigators /
Co-Supervisors
Institution
University of Leicester
Department
Biology
Funding type
Research
Value (£)
195,672
Status
Completed
Type
Research Grant
Start date
02/05/2000
End date
01/02/2004
Duration
45 months
Abstract
The research will enhance understanding of mechanisms for importing nucleus- encoded proteins into chloroplasts, and will focus on the outer envelope membrane Toc complex. The ppil mutant (lacking Toc33) is a unique resource (Science, 1998, 282: 100-103) that will be exploited fully. Four different approaches will be employed: (1) The import defect of ppil will be characterised in detail, elucidating the specific role played by Toc33; (2) Antibodies against Toc33 and Toc34 will be generated and used in immunoprecipitation experiments to investigate the composition of Toc complexes; (3) Reverse screens for mutants lacking other Toc proteins (Toc 34, 75, 86) will be conducted. Characterisation of single and double mutants will illuminate Toc functionality; and (4) A ppil screen will be conducted; this should identify new translocon components.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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