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Molecular dissection of the jasmonate perception-response pathway in Arabidopsis

Reference	P12879
Principal Investigator / Supervisor	Professor John Turner
Co-Investigators / Co-Supervisors	Dr Mark John Coleman
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	203,356
Status	Completed
Type	Research Grant
Start date	01/06/2000
End date	01/06/2003
Duration	36 months

Abstract

The COI1 gene is required for jasmonate (JA)-regulated defence and fertility in Arabidopsis. We isolated COI1, and predicted it codes for an F-box protein which recruits repressors of JA responses to ubiquitin-mediated proteolysis. To test this we used COI1 as bait in a yeast 2- hybrid screen of an Arabidopsis cDNA library, and isolated six interacting clones. Three are candidate repressors for COI1-targeted ubiquitination, and three are SKP1s, components of the ubiquitination complex. We propose to test the function of these COI1- interacting proteins in Arabidopsis, by down-regulation and by co-immunoprecipitation. We shall also identify other genes in the JA pathway by mutation, positional cloning, and by a new 2- hybrid screen.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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