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Virus-induced patterns of gene expression at single cell resolution in Arabidopsis
Reference
P12855
Principal Investigator / Supervisor
Dr Joel Milner
Co-Investigators /
Co-Supervisors
Dr Mark Hooks
,
Professor Deri Tomos
Institution
University of Glasgow
Department
IBLS Division of Biochemistry & Molecula
Funding type
Research
Value (£)
216,437
Status
Completed
Type
Research Grant
Start date
06/06/2000
End date
05/09/2004
Duration
51 months
Abstract
Symptom expression in virus-infected plants involves a programmed pattern of changes in gene expression. We have previously identified many genes whose expression is regulated at a whole plant level during CaMV infection of Arabidopsis. Using micro- capillaries, we will sample RNA from individual cells, and measure abundance of individual viral and host mRNAs using RT-PCR. We will develop a novel macro-array hybridisation and measure simultaneously, the levels of multiple transcripts in single cells. Patterns of gene expression in symptomatic tissue will be determined. Virus variants, and host symptom- response mutants, will be used to determine the influence of virus and host genotype on virus-inducible alterations in gene expression.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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