Award details

Nramps; potential transporters for essential divalent cations

Reference	P12786
Principal Investigator / Supervisor	Professor J.L Hall
Co-Investigators / Co-Supervisors	Dr Lorraine Williams
Institution	University of Southampton
Department	Centre for Biological Sciences
Funding type	Research
Value (£)	199,368
Status	Completed
Type	Research Grant
Start date	05/01/2000
End date	04/01/2004
Duration	48 months

Abstract

This proposal aims to use molecular and biochemical techniques in the model plant Arabidopsis thaliana to establish the role of a novel and potentially very important group of membrane transporters, the Nramp family. This gene family has only recently been identified in higher plants and evidence from other species suggests that members of the family may play a key role in the acquisition and homeostasis of essential divalent cations. A PCR- based reverse genetics approach will be used to identify insertional mutations in Nramp genes in individual plants. The resulting phenotypes will be analysed by physiological and biochemical experiments. Additional information relating to function will be obtained by investigating tissue and cellular expression and localisation of Nramps using promoter-GUS fusions, Northern analysis and Western blotting.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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