BBSRC Portfolio Analyser
Award details
The mating pheromones and receptors of C. cinereus; a model system for studying G-protein-coupled receptors
Reference
P12736
Principal Investigator / Supervisor
Dr L Casselton
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Plant Sciences
Funding type
Research
Value (£)
168,044
Status
Completed
Type
Research Grant
Start date
03/07/2000
End date
02/07/2003
Duration
36 months
Abstract
Sexual development in C. cinereus is regulated by a large family of functionally redundant pheromones and G-protein-coupled receptors (GPCRs) encoded by the B mating type genes. We wish to exploit the natural variability of this family of wild type signalling molecules to learn more about the biology of a ubiquitous class of proteins. Sequence alignments can identify conserved regions that permit us to predict structure-function relationships. By expressing pheromones and receptors in yeast, we show that pheromones may act as agonists and inverse agonists and we can distinguish between different causes of constitutive signalling. We plan to optimise our functional assays to better study these phenomena. We also plan to localise the receptors in C. cinereus cells since signalling appears to be confined within the fungal hyphae.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search