Award details

The role of a delta-like PI-phospholipase C in guard cell signalling

Reference	P12154
Principal Investigator / Supervisor	Professor Alistair Hetherington
Co-Investigators / Co-Supervisors	Professor Julie Gray, Dr Martin Mcainsh
Institution	Lancaster University
Department	Lancaster Environment Centre
Funding type	Research
Value (£)	95,455
Status	Completed
Type	Research Grant
Start date	01/09/1999
End date	01/09/2002
Duration	36 months

Abstract

We have recently cloned a guard cell phosphoinositide-specific phospholipase C (PI- PLC) gene. When the expression of this gene is manipulated the phenotype of the resulting plants is consistent with an impairment in the ability of the guard cells to respond to extracellular stimuli. We wish to capitalise on these preliminary results and employ whole plant, cell physiological and molecular techniques to investigate thoroughly the contribution that PI-PLC makes to guard cell stimulus-response coupling. By characterising the response of these plants to stimuli known to influence stomatal turgor and gene expression (ABA, CO2, light, extracellular calcium) we shall determine which stimulus-response coupling pathways utilise PI-PLC. We shall also use ratio photometry to investigate the contribution that PI-PLC makes to defining stimulus- induced increases in cytosolic free calcium and hence address the question of specificity. In addition as there is evidence that the expression of plant PI-PLCs can be regulated by environmental signals we shall determine whether the expression of a PI-PLC gene is controlled by extracellular stimuli in guard cells. (Joint with grant 50/P11544)

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

P11544 The role of a delta-like PI- phospholipase C in guard cell signalling

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