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Functional analysis of a higher plant putative calcium channel
Reference
P11983
Principal Investigator / Supervisor
Professor Dale Sanders
Co-Investigators /
Co-Supervisors
Institution
University of York
Department
Biology
Funding type
Research
Value (£)
171,980
Status
Completed
Type
Research Grant
Start date
16/09/1999
End date
01/03/2003
Duration
41 months
Abstract
A putative CA2+ channel has recently been sequenced as part of the Arabidopsis genome project. This gives us - for the first time - the opportunity to address the molecular basis of calcium signal generation in plants. The channel will be characterised functionally after heterologous expression and, in transient expression studies, assessed for its ability to evoke changes in cytosolic free CA2+ in response to stress stimuli. The expression pattern will be mapped to yield clues to function, and physiological function assessed more directly by overexpression and the analysis of knock-out mutants identified by reverse genetic approaches. The molecular basis of the regulation of channel activity will be assessed with mutants in which key regions of the protein thought to be involved in voltage- sensing and control by phosphorylation have been modified.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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