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Award details
Studies on the KpsE protein and its role in the transport of group II capsular polysaccharides in Escherichia coli
Reference
P11944
Principal Investigator / Supervisor
Professor Ian Roberts
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
192,866
Status
Completed
Type
Research Grant
Start date
01/01/2000
End date
01/06/2003
Duration
41 months
Abstract
The proposal will study the role of KpsE in the transport of group II capsular polysaccharides across the periplasmic space and to identify a role for a trans-periplasmic transport complex. Initially a structure/function analysis of KpsE will determine if the C-terminal amphipathic helix mediates interactions with the inner-membrane, determine the ability of KpsE to form a coiled-coil and establish if the N-terminal membrane-spanning domain interacts with the capsule biosynthetic complex on the inner membrane. Subsequently cross-linking with affinity chromatography will be used to direct the protein/protein and protein/polysaccharide interactions involved in polysaccharide transport and to study the trans- periplasmic export complex.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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