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Characterisation of a light-dependent trans-cis isomerases involved in umbelliferone biosynthesis in pea
Reference
P11627
Principal Investigator / Supervisor
Dr Mark Barber
Co-Investigators /
Co-Supervisors
Professor Timothy Bugg
Institution
University of Southampton
Department
Centre for Biological Sciences
Funding type
Research
Value (£)
93,968
Status
Completed
Type
Research Grant
Start date
21/02/2000
End date
20/08/2002
Duration
30 months
Abstract
The current proposal challenges the currently accepted mechanism for coumarin biosynthesis in plants. We hypothesise that in addition to photoisomerism the trans-cis isomerisation of the alpha-beta double bond of the aliphatic side chain of trans 2- hydroxycinnamic acids is mediated by specific trans-cis isomerases. It is proposed to characterise the trans-cis isomerases responsible for the trans-cis isomerisation of trans 2,4- dihydroxycinnamic acid (2,4-DCA) in relation to the synthesis of umbelliferone in pea. Characterisation will focus on the physical properties of the trans-cis isomerases, their substrate specificity's and cofactor requirements. In particular, a series of experiments are planed to elucidate the likely enzymic mechanisms operating for the light-independent and light-dependent forms of trans-cis isomerase.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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