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Characterisation of a light-dependent trans-cis isomerases involved in umbelliferone biosynthesis in pea

ReferenceP11627
Principal Investigator / Supervisor Dr Mark Barber
Co-Investigators /
Co-Supervisors
Professor Timothy Bugg
Institution University of Southampton
DepartmentCentre for Biological Sciences
Funding typeResearch
Value (£) 93,968
StatusCompleted
TypeResearch Grant
Start date 21/02/2000
End date 20/08/2002
Duration30 months

Abstract

The current proposal challenges the currently accepted mechanism for coumarin biosynthesis in plants. We hypothesise that in addition to photoisomerism the trans-cis isomerisation of the alpha-beta double bond of the aliphatic side chain of trans 2- hydroxycinnamic acids is mediated by specific trans-cis isomerases. It is proposed to characterise the trans-cis isomerases responsible for the trans-cis isomerisation of trans 2,4- dihydroxycinnamic acid (2,4-DCA) in relation to the synthesis of umbelliferone in pea. Characterisation will focus on the physical properties of the trans-cis isomerases, their substrate specificity's and cofactor requirements. In particular, a series of experiments are planed to elucidate the likely enzymic mechanisms operating for the light-independent and light-dependent forms of trans-cis isomerase.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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