Award details

Studies of expansin structure and function

ReferenceP11582
Principal Investigator / Supervisor Professor Simon McQueen-Mason
Co-Investigators /
Co-Supervisors
Institution University of York
DepartmentBiology
Funding typeResearch
Value (£) 208,700
StatusCompleted
TypeResearch Grant
Start date 01/09/1999
End date 01/09/2002
Duration36 months

Abstract

The work will cover a number of areas related to expansin structure and function. We have recently produced active recombinant expansins in cultured insect cells, and in transgenic plants. This opens up new and important opportunities to study these unique enzymes. Using site-directed mutagenesis we will explore the role of particular residues and motifs in the binding and activity of expansins. Recently, expansin isoforms have been implicated in a number of novel biological processes. We will express expansin cDNAs from a variety of different plants and tissues and characterise the activity and substrate specificity of these isoforms. We will develop assays to determine whether expansin action involves polymer hydrolysis or hydrogen bond disruption, and whether expansin interacts with XET in the cell wall.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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