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Chloroplast interconnections via stromules
Reference
P11561
Principal Investigator / Supervisor
Professor John Gray
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Plant Sciences
Funding type
Research
Value (£)
176,853
Status
Completed
Type
Research Grant
Start date
01/05/1999
End date
01/11/2002
Duration
42 months
Abstract
The aim is to understand the mechanism of stromule formation between chloroplasts and to determine the size limits for movement of proteins and nucleic acid from chloroplast to chloroplast via stromules. The presence of envelope membranes in the stromules will be examined with green fluorescent protein fusions to targeting sequences for the inner and outer envelope membranes in transgenic tobacco plants. The size limit for protein movement will be investigated using GFP fusions to stromal proteins, including subunits of Rubisco and ribosomes, and localised two-photon photobleaching. Movement of the nucleic acids will be examined following microinjection of fluorescent-labelled RNA and DNA into single chloroplasts. The possible involvement of an actin-based motor system will be examined biochemically.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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