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Using single cell sampling to localise transcriptional control of primary carbohydrate metabolism in temperate gramineae

Reference	P11556
Principal Investigator / Supervisor	Professor Deri Tomos
Co-Investigators / Co-Supervisors	Professor John Farrar, Dr Olga Koroleva, Professor Chris Pollock
Institution	Bangor University
Department	Sch of Biological Sciences
Funding type	Research
Value (£)	207,493
Status	Completed
Type	Research Grant
Start date	15/12/1999
End date	15/12/2002
Duration	36 months

Abstract

The cereal leaf is a multi-tissue organ which fixes carbon in photosynthesis and exports sucrose to the phloem. We have already shown how different cell types have specialised roles in leaf carbohydrate metabolism. Now we shall measure the transcriptional basis of these differences by extracting from individual cells and tissues specific mRNA for enzymes in photosynthesis, sucrose and fructan metabolism, and starch metabolism. This will be done under conditions of low and high carbon flux rate and of high and low accumulation of sucrose and fructans. We shall determine dose-response curves and time courses for different control elements of the signal transduction pathway.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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