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Characterisation of conserved viral strategies for infecting insect hosts

ReferenceP11541
Principal Investigator / Supervisor Dr Julie Olszewski
Co-Investigators /
Co-Supervisors
Institution Imperial College London
DepartmentBiological Sciences
Funding typeResearch
Value (£) 166,180
StatusCompleted
TypeResearch Grant
Start date 22/11/1999
End date 22/11/2002
Duration36 months

Abstract

GP37/fusolin and ORF145/150 related genes are found in two unrelated insect virus taxa, the baculoviruses and entomopoxviruses, suggesting they confer some broad advantage to viral pathogens utilising insect hosts. We aim to identify the function(s) of these genes as a first step towards elucidating mechanisms that are fundamental to the host/virus interaction. AcMNPV mutants lacking GP37, ORF145, ORF150 or both ORFs 145 & 150, will be characterised, and GP37 and HaEPV fusolin will be assayed for enhancing activity. Yeast 2-hybrid analysis will be used to identify proteins that interact with AcMNPV GP37, ORF145 & 150, and HaEPV fusolin & 11K protein, and antibodies raised to AcMNPV ORF145 & 150 will be used to study their expression.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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