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Nitrate reduction to ammonia by fermentative bacteria: major new roles for the periplasmic nitrate and nitrite reductases

Reference	P11528
Principal Investigator / Supervisor	Professor Jeffrey Cole
Co-Investigators / Co-Supervisors	Professor David Richardson
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	302,341
Status	Completed
Type	Research Grant
Start date	01/09/1999
End date	01/09/2002
Duration	36 months

Abstract

Fermentative bacteria synthesise two active nitrate reductases, the cytoplasmic NarA and Nap in the periplasm. Nitrite, the product of nitrate reduction, is reduced to ammonia b a periplasmic nitrite reductase, Nrf. The five objectives of this research are to: define the regulation of each of the three nitrate reductases and their contributions to the overall rate of nitrate reduction under different growth conditions, establish whether the affinity of Nap and Nrf are consistent with a physiological role in nitrate and nitrite reduction at low oxy-anion concentrations; determine which of the Nap components are essential for nitrate reduction by Nap under different growth conditions; characterise the NapF, G and H proteins by a combination of magneto-optical spectroscopies and electrochemistry; characterise the NrfB, C and D proteins and determine their role in electron transfer from quinols to NrfA.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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