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The biochemical nature of overexpression of mtHSP70 on plant growth and development in tobacco
Reference
P11487
Principal Investigator / Supervisor
Dr Carlton Wood
Co-Investigators /
Co-Supervisors
Professor Anthony Moore
Institution
University of Sussex
Department
Sch of Life Sciences
Funding type
Research
Value (£)
173,427
Status
Completed
Type
Research Grant
Start date
01/06/1999
End date
01/06/2002
Duration
36 months
Abstract
The objectives are to investigate the biochemical nature of the increased growth traits observed in transgenic tobacco over-expressing PHSP1, in particular to test the hypothesis that the overexpression of this mtHSP70 has resulted in an increased availability of carbon skeletons (in the form of citrate) for biosynthesis and nitrogen assimilation. This will be done using transgenic lines 32 and 25 by (1) analysing the rate and capacity of the protein import machinery; (2) determining (a) the capacity and activity of the respiratory chain for NADH re-oxidation, (b) in vivo respiratory activity by IRGA and (c) the capacity and activity of the tricarboxylate carrier; (3) quantitatively assessing mitrochondrial structure and numbers per cell; (4) quantification of metabolites by 13C and 31P NMR.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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