BBSRC Portfolio Analyser
Award details
Insecticidal Photorhabdus toxins as alternatives to Bt
Reference
P11446
Principal Investigator / Supervisor
Professor Richard Ffrench-Constant
Co-Investigators /
Co-Supervisors
Institution
University of Bath
Department
Biology and Biochemistry
Funding type
Research
Value (£)
209,209
Status
Completed
Type
Research Grant
Start date
01/10/1999
End date
01/10/2002
Duration
36 months
Abstract
The long term goal of this project is to provide insecticidal protein toxins for use as alternatives to those derived from Bacillus thuringiensis or Bt. These would be either expressed in transgenic crops or used in conventional sprays or baits. In preliminary experiments we have cloned and sequenced four toxin complex genes from the bacteria Photorhabdus luminescens which each encode an insecticidal protein complex of high molecular weight (termed Photorhabdus toxins or Pht). Three years worth of research is therefore proposed to answer the following questions: (1) what is the effect of knocking out each of these toxin complex encoding loci individually or in combination? (2) what are the minimal effective toxin units suitable for plant expression or toxin production? (3) can we functionally express P. luminescens toxin or in other bacteria such as E. coli? If not, what is lacking? The appropriate secretion machinery and/or the normal proteolytic cleavage observed in P. luminescens broth.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search