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Simultaneous imaging of fungal and plant carbon metabolism during pathogenesis

Reference	P11215
Principal Investigator / Supervisor	Professor Julie Scholes
Co-Investigators / Co-Supervisors	Dr Paul Bowyer, Professor Stephen Rolfe
Institution	University of Sheffield
Department	Animal and Plant Sciences
Funding type	Research
Value (£)	218,680
Status	Completed
Type	Research Grant
Start date	01/02/1999
End date	01/10/2002
Duration	44 months

Abstract

We propose to take a novel approach to study the interaction between wheat and two economically important pathogens Septoria nodorum and Mycosphaerella graminicola. We will use real-time, non-invasive imaging of host chlorophyll fluorescence as a sensitive measure of photosynthetic metabolism. Simultaneous imaging of fungi transformed with Green Fluorescent Protein will allow the location of the hyphae to be determined non- destructively and, when fused to promoters induced by specific carbon compound, act as a sensitive measure of fungal carbon metabolism. Specifically we will ask: (i) What is the relationship between the location of the fungal mycelium, visible symptoms and alterations in the photosynthetic metabolism of the host during pathogenesis? (ii) How does the carbon metabolism of the pathogen alter during the different nutritional phases of its life cycle? (iii) Does the switch to necrotrophic growth occur at a particular stage of fungal development and within the leaf or is it associated with a particular nutritional state of the fungus?

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	BioImaging (BI) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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