Award details

An enhanced FRAP facility on the Daresbury Laboratory confocal microscope for the study of membrane dynamics in cyanobacteria

Reference	P11202
Principal Investigator / Supervisor	Dr M Tobin
Co-Investigators / Co-Supervisors	Professor Conrad Mullineaux
Institution	STFC - Laboratories
Department	STFC - Laboratories Department
Funding type	Research
Value (£)	29,440
Status	Completed
Type	Research Grant
Start date	25/06/1999
End date	25/06/2000
Duration	12 months

Abstract

The confocal microscope facility at Daresbury Laboratory will be developed to provide a highly flexible FRAP instrument targeted specifically at the ongoing studies of membrane mobility of photosynthetic complex molecules in cyanobacteria. The current sample stage will be replaced by a conventional fluorescence microscope, and a high speed shutter, synchronised with the scanning mirrors will be used to enable bleaching of specific selected regions of the sample. A fibre optic system will be used to allow the sequential FRAP study of more than one component in single bacterial cells. These modifications will allow the study of features such as thylakoid membrane continuity and the process of intracellular communication at much higher spatial resolution than is currently possible with our system.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	BioImaging (BI) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search