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Molecular analysis of RpoI a putative regulatory sigma factor for symbiotic nitrogen fixation and iron uptake by Rhizobium

Reference	P10999
Principal Investigator / Supervisor	Professor Andrew Johnston
Co-Investigators / Co-Supervisors
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	155,108
Status	Completed
Type	Research Grant
Start date	05/07/1999
End date	01/08/2003
Duration	49 months

Abstract

We have identified a gene, rpoI, in Rhizobium leguminosarum the symbiont of peas which is involved in siderophore production and N2 fixation. The sequence of RpoI indicates that it is a member of the family of ECF sigma factors, being most closely related to the PvdS protein of Pseudomonas. Experiments are described to: (i) see if RpoI is functionally equivalent to PodS and if it is regulated by fur; (ii) use reporter fusion mutagenesis to see what genes, particularly those involved in symbiotic N2 fixation, are regulated by RpoI; (iii) demonstrate biochemically and genetically if RpoI is a sigma factor; (iv) characterise nodule developments in rpoI mutants; (v) study the factors that regulate rpoI expression.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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