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Discovering the interface between the plant cell cycle plant growth regulators and development with cdc25

Reference	P10942
Principal Investigator / Supervisor	Dr Dennis Francis
Co-Investigators / Co-Supervisors	Dr Richard Dickinson, Professor Nigel Halford, Professor Hilary Joan Rogers
Institution	Cardiff University
Department	School of Biosciences
Funding type	Research
Value (£)	184,527
Status	Completed
Type	Research Grant
Start date	01/04/1999
End date	01/08/2002
Duration	40 months

Abstract

Induction of fission yeast cdc25 transcripts in tobacco led to more, smaller lateral roots which comprised smaller cells compared with the wild type. The smaller cell size stabilised indicating that the Cdc25 phosphatase was down-regulated by plant cell cycle proteins. What are these regulatory proteins and how do they, and the Cdc25 phosphatase cause these developmental changes? Using cdc25 as bait in yeast two hybrid (2H) assays in Arabidopsis we shall isolate cDNAs encoding putative regulators of Cdc25. These will be used to transform Arabidopsis in which we will examine lateral root size and frequency. We shall also transform IAA (alf) mutants defective in lateral root morphogenesis to test the hypotheses that cdc25 is at the end of a signal transduction chain which initiates laterals. This would link cell cycle regulatory genes with an IAA-mediated developmental event.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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