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Award details
Molecular characterisation of the genes and enzymes of the gentisate pathway for aromatic catabolism
Reference
P10381
Principal Investigator / Supervisor
Professor Peter Williams
Co-Investigators /
Co-Supervisors
Institution
Bangor University
Department
Sch of Biological Sciences
Funding type
Research
Value (£)
210,343
Status
Completed
Type
Research Grant
Start date
01/02/1999
End date
15/09/2002
Duration
44 months
Abstract
The nag genes encoding the catabolism of naphthalene through gentisate in a Pseudomonas, strain U2 will be cloned and sequenced in their entirety. The complete pathway will be characterised by reconstructing the pathway in a vector and inactivating individual genes. All the genes for the pathway reactions will be individually expressed and assayed to characterise each metabolic step. A monooxygenase with novel structure, salicylate 5-hydroxylase, will be expressed and its reaction specificity against a wide variety of aromatic substrates determined. The evolutionary potential of the early genes of the nag cluster to attack nitrotoluenes will be assessed and any mutations with this acquired modified substrate specificity will be sequenced.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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