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Studies on group II capsular polysaccharide biosynthesis in Escherichia coli
Reference
P10360
Principal Investigator / Supervisor
Professor Ian Roberts
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
200,152
Status
Completed
Type
Research Grant
Start date
01/02/1999
End date
01/02/2002
Duration
36 months
Abstract
The proposal will use multi-disciplinary approaches to elucidate the biosynthesis of group II capsular polysaccharides in Escherichia coli. The specific aims are: First to identify the alpha-glycosyltransferase active site in the KfiC transferase which polymerises the K5 polysaccharide. KfiC is a rare example of a glycosyltransferase with both alpha and beta transferase activity. Such bifunctional glycosyltransferases have not been studied to date. Secondly to identify the roles of the KpsC and S proteins in the attachment of phosphatidyl-KDO to the reducing end of group II capsular polysaccharides. Finally to elucidate the protein/protein and protein/polysaccharide interactions in the assembly and function of the polysaccharide biosynthetic complex on the inner membrane. This study will reveal at the molecular level the biosynthesis of capsular polysaccharides in E. coli.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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