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Responses of Escherichia coli to zinc stress: essentiality versus toxicity

Reference	P10354
Principal Investigator / Supervisor	Professor Robert K Poole
Co-Investigators / Co-Supervisors	Professor M Hughes, Professor Cameron McLeod
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	219,476
Status	Completed
Type	Research Grant
Start date	09/11/1998
End date	08/04/2004
Duration	65 months

Abstract

Zinc is an essential element for the growth of microbes, plants and animals, but virtually nothing is known of the mechanisms of zinc-uptake and regulation of intracellular Zn pools. We propose a) to determine how Zn is transported into and out of Escherichia coli by exploiting transposon mutants and novel analytical methods for metals; b) to determine how E. coli responds to Zn stress, i.e. either Zn excess or Zn limitation, at the levels of gene expression and protein synthesis; c) to examine the subcellular distributions of Zn, d) to seek additional genes responsive to Zn stress, and e) to initiate studies of the organisation and structure of ZntA, a P-type metal cation- transporting ATPase.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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