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Characterisation of the Arabidopsis genes RPW7 and RPW8 required for resistance to two powdery mildew diseases
Reference
P10348
Principal Investigator / Supervisor
Professor John Turner
Co-Investigators /
Co-Supervisors
Dr Mark John Coleman
Institution
University of East Anglia
Department
Biological Sciences
Funding type
Research
Value (£)
224,101
Status
Completed
Type
Research Grant
Start date
01/08/1998
End date
01/08/2001
Duration
36 months
Abstract
The loci RPW7 and RPW8 control resistance of Arabidopsis thaliana ecotype Ms-0 to powdery mildew diseases caused by Erysiphe cruciferarum UEA1 and E. cichoracearum UCSC1 respectively. RPW7 and RPW8 map genetically and physically to a 42 kb Ms-0 cosmid contig. Plants transformed with one of these cosmids, 612B6, are resistant to infection. We shall determine the DNA sequence of cosmid 612B6, clone ORFs defined by sequence, and transfer these ORF's to susceptible plants to test for R-genes. The defence signalling pathways regulated by RPW7/RPW8 will be characterised by testing for RPW7/RPW8-mediated disease resistance in genetic backgrounds containing eds1, coi1, ndr1, and nar1. To evaluate their expression in other species, RPW8 will be transferred to squash (which is susceptible to UCSC1), and RPW7 will be transferred to Brassica napus (which is susceptible to UEA1) and transformants will be tested for disease resistance. To isolate genes for proteins of plant or fungal origin that interact with RPW8, we shall use the yeast 2-hybrid system with RPW8 as bait to screen for interacting clones in a cDNA library made form diseased Arabidopsis leaves.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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