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Mechanistic studies of nitrate and nitrite transport; involvement of NarK and regulation by oxygen

Reference	P10251
Principal Investigator / Supervisor	Professor James Moir
Co-Investigators / Co-Supervisors	Professor Stuart John Ferguson, Professor David Richardson
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	135,030
Status	Completed
Type	Research Grant
Start date	01/02/1999
End date	01/02/2002
Duration	36 months

Abstract

Nitrate reduction by the bacterial membrane-bound dissimilatory nitrate reductase requires a specific transporter to move nitrate across the cytoplasmic membrane. We do not yet know the mechanism of nitrate transport but consider that the protein NarK is involved in the process. We have constructed deletion mutants in NarK from the denitrifier Paracoccus denitrificans and have designed a set of experiments to identify the role of NarK in the transport of nitrate and/or nitrite. We will investigate both the inhibition of nitrate transport and reduction by oxygen and the role of oxygen in regulation of nitrate reductase gene expression, mediated via a recently discovered regulator NarR. Programmes of random and site-directed mutagenesis will also be undertaken in order to determine more clearly the cellular and molecular mechanisms of nitrate and nitrite transport.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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