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The role of novel L-galactose metabolising enzymes in ascorbate biosynthesis by plants
Reference
P10241
Principal Investigator / Supervisor
Professor Nicholas Smirnoff
Co-Investigators /
Co-Supervisors
Institution
University of Exeter
Department
Biosciences
Funding type
Research
Value (£)
216,802
Status
Completed
Type
Research Grant
Start date
04/01/1999
End date
04/01/2002
Duration
36 months
Abstract
Ascorbate is a key antioxidant in plants and its provides the major source of vitamin C in the human diet. We have recently proposed the first complete biosynthetic pathway for L- ascorbic acid in plants. It includes a newly-discovered enzyme L-galactose dehydrogenase, which forms L-galactono-1,4-lactone. This is oxidised to ascorbate by L- galactono-1,4- lactone dehydrogenase. L-galactose is formed from GDP-L-galactose by unknown enzymes. The aim of this proposal is to characterise novel L-galactose metabolising enzymes involved in ascorbate metabolism and determine their roles in controlling ascorbate synthesis. Specific objectives are: 1. Purify and clone the novel L-galactose dehydrogenase with the ultimate objective of defining its role in transgenic plants. 2. Identify the enzyme(s) which synthesise L-galactose from GDP-galactose.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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