Award details

Molecular and biochemical analysis of the distinctive organelle-specific plant pyruvate dehydrogenase complexes

Reference	P10229
Principal Investigator / Supervisor	Professor John Lindsay
Co-Investigators / Co-Supervisors	Professor Richard Cogdell
Institution	University of Glasgow
Department	IBLS Division of Biochemistry & Molecula
Funding type	Research
Value (£)	199,096
Status	Completed
Type	Research Grant
Start date	01/01/1999
End date	01/01/2003
Duration	48 months

Abstract

a) purified chloroplastic E2, expressed as a GST fusion protein and its overexpressed N-terminal didomain will be employed i) as probes to examine the specificity, affinity and stoichiometry of E2 interactions with its companion E1/E3 components and ii) in 'pull-down' assays to detect candidate proteins such as antioxidant or other stress- related proteins which form physiologically relevant interactions with the complex; b) characterisation of plastidic E3 cDNA clones will be completed; c) the developmental regulation of mitochondrial and plastidic E3s will be monitored at transcriptional, translational and activity levels; d) characterisation of clones encoding the alpha- isoform of potato mitochondrial E3 will be performed as will structure-function analysis of the enzymatic properties and precise physiological roles of the 3 distinct E3 isoforms.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search