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Functional analysis of the receptor-ligand interaction in the SI reaction of Papaver rhoeas

Reference	P10108
Principal Investigator / Supervisor	Professor Chris Franklin
Co-Investigators / Co-Supervisors	Professor Vernonica E. (Noni) Franklin-Tong, Dr Jonathan Paul Ride
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	201,579
Status	Completed
Type	Research Grant
Start date	01/05/1999
End date	01/12/2002
Duration	43 months

Abstract

The aims of this project are to determine the amino acid residues in the stigmatic S- protein of Papaver rhoeas that play a key role in the recognition of incompatible pollen using site-directed mutagenesis. A series of mutants will be constructed with the express objectives of: [A] Identification of S-protein mutants defective in receptor recognition. This will encompass: 1) defining the residues which interact with the recently identified accessory receptor (SBP)-binding region and 2) the residues that participate in S-allele specific pollen recognition. We also intend to complement the mutation analysis by undertaking: [B] structural analysis of a stigmatic S-protein, using crystallographic or NMR approaches, and [C] studies of the interaction of stigmatic S-proteins with the pollen S-receptor.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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