Award details

Characterisation and export of the novel Escherichia coli haemolysin HlyE

Reference	P09884
Principal Investigator / Supervisor	Professor J Green
Co-Investigators / Co-Supervisors	Professor J Guest
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	140,646
Status	Completed
Type	Research Grant
Start date	04/01/1999
End date	04/01/2002
Duration	36 months

Abstract

This proposal will initiate the characterisation of a unique family of haemolytic proteins by investigation of the properties and export of the E. coli hlyE gene product. The hlyE gene encodes a novel haemolysin and is a member of the FNR-modulon. Preliminary work has shown that active FNR is involved in both HlyE expression and export. The aims of this proposal are to: 1) determine the biochemical and biophysical properties of purified HlyE; 2) identify and study the regulation of the genes required for HlyE export under anaerobic conditions; 3) investigate structure- function relationships in HlyE in order to identify those regions of the protein associated with haemolytic activity and export.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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