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Structural and functional analysis of the Mlo protein family
Reference
P09868
Principal Investigator / Supervisor
Professor Sir David Baulcombe
Co-Investigators /
Co-Supervisors
Institution
University of East Anglia
Department
Sainsbury Laboratory
Funding type
Research
Value (£)
225,943
Status
Completed
Type
Research Grant
Start date
13/10/1998
End date
31/08/2002
Duration
47 months
Abstract
Molecular isolation of the Mlo gene, a negative regulator of pathogen defence and cell death in barley, revealed related genes in monocot and dicot plant species. Mlo appears to be the founder of a novel and abundant family of plant regulatory proteins which exhibit structural similarities to mammalian G protein coupled receptors. We propose a thorough structural and functional analysis of Mlo by applying protein topology and subcellular localisation studies. A novel transient transformation system will be employed to identify functional domains in Mlo by using site-directed mutagenesis and domain swaps between family members. A series of pharmacological compounds will be used to test the involvement of heterotrimeric G proteins in mlo-mediated defence with a simplified coleoptile monolayer tissue system.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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