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Control of specific mRNA stability by chilling temperatures in cereals
Reference
P09664
Principal Investigator / Supervisor
Professor Monica Hughes
Co-Investigators /
Co-Supervisors
Institution
Newcastle University
Department
School of Cell and Molecular Biosciences
Funding type
Research
Value (£)
188,471
Status
Completed
Type
Research Grant
Start date
01/09/1998
End date
01/09/2001
Duration
36 months
Abstract
Post-transcriptional mechanisms have been shown to be important in the environmental control of plant gene expression and are therefore involved in the environmental control of plant growth and development. The aim of this project is to isolate and characterise the gene(s) encoding the RNA-stabilising protein(s) responsible for regulating mRNA stability of a low-temperature-responsive barley gene (blt14). This will be achieved by (i) identifying the mRNA sequence element binding sites for stabilising proteins; (ii) using a yeast 3-hybrid cloning system to isolate gene(s) from an existing yeast low-temperature-barley shoot meristem cDNA library; (iii) sequencing and establishing the pattern of expression of the gene(s) and (iv) characterising the RNA-binding properties of the protein(s) encoded by the gene(s).
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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