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Characterisation of a nitric oxide responsive transcription factor
Reference
P09655
Principal Investigator / Supervisor
Dr S Spiro
Co-Investigators /
Co-Supervisors
Institution
University of East Anglia
Department
Biological Sciences
Funding type
Research
Value (£)
195,337
Status
Completed
Type
Research Grant
Start date
01/10/1998
End date
01/10/2001
Duration
36 months
Abstract
The transcription factor Nnr from Paracoccus denitrificans activates gene expression in response to nitric oxide. The biochemical properties of purified Nnr will be studied, specifically its oligomeric state, sulphydryl/disulphide content, metal content and spectroscopic properties, in the presence and absence of NO. Gel retardation assays will be used to measure the affinity of Nnr for cognate and non-cognate binding sites in DNA. RNA polymerase will be purified from P. denitrificans and used in run-off transcription assays, which will establish the sigma factor requirements of Nnr-regulated promoters. The effect of NO on DNA binding and transcription activation will be determined, in order to gain further understanding of the mechanism by which Nnr activity is modulated by NO.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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