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The first bacterial cytokine; production and properties

Reference	P09644
Principal Investigator / Supervisor	Professor Michael Young
Co-Investigators / Co-Supervisors	Professor Douglas Kell
Institution	Aberystwyth University
Department	Inst of Biological, Environ & Rural Sci
Funding type	Research
Value (£)	198,235
Status	Completed
Type	Research Grant
Start date	24/08/1998
End date	24/12/2001
Duration	40 months

Abstract

We shall exploit our breakthrough finding that dormant, non-growing cells of M. luteus can be resuscitated in growth medium only by the addition of picomolar concentrations of an autocrine proteinaceous growth factor (Rpf), which shows strong similarity to predicted gene products in Mycobacterium tuberculosis and Mycobacterium leprae. We shall do this by over-expressing this and a related M. luteus protein in E. coli and characterising them biochemically. We shall develop quantitative assays for their activity and establish their specificity and production kinetics, whether the secreted gene product is subjected to specific proteolytic cleavage, and whether any such cleavage leads to the modification of biological activity.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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