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Structural studies of the higher plant photosystem I complex using electron crystallography

Reference	P09559
Principal Investigator / Supervisor	Professor Robert Ford
Co-Investigators / Co-Supervisors	Professor Andreas Kurt Hans Holzenburg
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	167,906
Status	Completed
Type	Research Grant
Start date	10/08/1998
End date	10/12/2001
Duration	40 months

Abstract

We propose to study the structure of higher plant photosystem I (PSI) using electron microscopy of two-dimensional (2-D) crystals of the complex. These crystals are the first reported for the higher plant complex, and they open up the possibility of obtaining much more structural data, allowing it's comparison with the simpler cyanobacterial PSI complex. A 3-D structure will be obtained for negatively- stained crystals, and then cryo-electron microscopy of unstained specimens will be carried out in order to extend the resolution of the structural data beyond 2.0 nm. Mapping of the locations of the higher plant PSI subunits will be attempted by antibody labelling; salt and chaotrope washing; and by the use of various mutants. The binding sites for soluble electron transfer proteins on the complex will also be investigated by electron microscopy.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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