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Molecular genetic dissection of virus symptom phenotypes elicited in transgenic Arabidopsis plants

Reference	P09461
Principal Investigator / Supervisor	Dr Joel Milner
Co-Investigators / Co-Supervisors
Institution	University of Glasgow
Department	IBLS Division of Biochemistry & Molecula
Funding type	Research
Value (£)	204,274
Status	Completed
Type	Research Grant
Start date	01/05/1998
End date	01/11/2003
Duration	66 months

Abstract

Transgenic Arabidopsis expressing cauliflower mosaic virus protein P6 from 3 different virus strains exhibit a range of symptom-like phenotypes, and provide a model system for investigating host-virus interactions in symptom expression. Seeds from transgenic lines will be mutagenised, and the progeny screened for mutants with altered phenotype severity. We aim to identify mutants that do not map in the transgene, i.e. in host loci controlling or involved in symptom expression. We have already identified one such mutant, which is insensitive to P6. This, together with further mutants, will be characterised genetically, and where possible cloned and sequenced. Differential display PCR will be used to elucidate the effects of gene VI expression and virus replication on patterns of host gene expression. The effects on the patterns of gene expression within the mutants will be analysed.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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