Award details

The mechanism of signal transduction in a novel chemotaxis system essential for virulence in Helicobacter pylori 36

Reference	P09432
Principal Investigator / Supervisor	Professor Dave Kelly
Co-Investigators / Co-Supervisors
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	192,142
Status	Completed
Type	Research Grant
Start date	01/09/1998
End date	01/09/2001
Duration	36 months

Abstract

Helicobacter pylori is one of the commonest infections in man and is the causative agent of type B gastritis and gastric and duodenal ulcer disease. Although the nature of the signals that attract H. pylori into the gastric mucosa are largely unknown, chemotaxis is likely to be an important factor for successful mucosal colonisation. We have found that H. pylori contains a novel set of chemotaxis genes and, through mutant studies, that chemotaxis is essential for virulence. In this proposal we will address the mechanism of signal transduction in this system, which consists of four MCPs, a CheW homologue, 3 homologues of Bacillus subtilis CheV, an bifunctional protein containing both CheA and CheY domains (designated CheF) and a separate CheY protein. We will (1) Purify overexpressed CheV, CheY, CheF and its separate CheA and CheY domains, in order to define the pathway of phosphotransfer in the system, by in vitro phosphorylation analyses. (2) Interactions between the proteins will be studied by gel filtration, PAGE and antibody studies. (3) CheW and CheV will be mutated to determine if they are functionally redundant. (4) The roles of the MCPs will be studied by mutagenesis and (5) wild-type and mutant cells will be analysed by video-tracking analysis.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search