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Identification of chemosensory receptors in Rhodobacter sphaeroides and control of their transcription and localisation
Reference
P09327
Principal Investigator / Supervisor
Professor Judith Armitage
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Biochemistry
Funding type
Research
Value (£)
245,010
Status
Completed
Type
Research Grant
Start date
01/06/1998
End date
01/06/2001
Duration
36 months
Abstract
Methyl accepting chemotaxis proteins have been identified in R. sphaeroides. One is targeted to previous septation sites under heterotrophic growth conditions, while a second constitutive MCP is localised cytoplasmically. 1. The genes for these MCPs will be cloned and sequenced. 2. They will be mutated in frame and the ones targeted to different locations identified by immunoelectron microscopy. 3. The sequences will be compared and the possible sites involved in targeting identified and altered by site directed mutagenesis. 4. Transcriptional and translational fusions will be made and their expression under different environmental control identified. 5. Signals controlling expression will be identified by transferring known mutations in oxygen sensing proteins into the R. sphaeroides cells carrying fusions. 6. Sites acting as targets will be characterised.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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