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Proteases as pathogenicity determinants of the cereal pathogen Stagonospora (Septoria) nodorum

Reference	P09299
Principal Investigator / Supervisor	Dr Richard Cooper
Co-Investigators / Co-Supervisors
Institution	University of Bath
Department	Biology and Biochemistry
Funding type	Research
Value (£)	198,983
Status	Completed
Type	Research Grant
Start date	01/04/1998
End date	01/05/2002
Duration	49 months

Abstract

Stagonospora nodorum produces on host cell walls a wide range of extracellular depolymerases; various evidence implicates proteases in infection. We have identified two aspartic and a single form of a trypsin-like protease. The trypsin has been purified, fully characterised (pI 8.9, Mr 30kDa, Phe-Val-Arg[NA] substrate) N-terminal sequence determined and partial nucleotide sequence obtained. This enzyme is present in pycnidial mucilage, developing lesions and infection is inhibited by the trypsin inhibitor aprotinin. The aspartic protease isoforms have been partially characterised and nucleotide sequence derived from a PCR product of S. nodorum genomic DNA. We aim to (1) complete sequencing of the protease genes (2) disrupt these and test pathogenicity (3) localise proteases in planta (4) assess effects of inhibitors and antibodies on infection (5) investigate activity of proteases on native and extracted cell wall proteins, and toxicity to host cells and protoplasts (6) overexpress protease cDNAs as a prelude to developing a rapid throughput screen for novel inhibitors.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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