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Genetic manipulation of the light harvesting antenna of green plants

Reference	P08723
Principal Investigator / Supervisor	Professor Peter Horton
Co-Investigators / Co-Supervisors	Professor Julie Gray
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	139,342
Status	Completed
Type	Research Grant
Start date	01/09/1998
End date	31/08/2000
Duration	24 months

Abstract

The light harvesting antenna of plants comprises 10 different chlorophylls a/b binding proteins encoded by the Lhcal-4 and Lhcbl-6 genes. With one exception, these proteins occur in low amounts, 1-2 per reaction centre. This heterogeneity has not been explained. It is proposed to use genetic manipulation to determine the function of each of the Lhc proteins. Levels of Lhc proteins in Arabidopsis thaliana will be altered by expression of antisense Lhc genes and by PCR screening of T-DNA insert lines to isolate mutants deficient in particular proteins. Transformed and mutant plants will be examined using biochemical methods to investigate thylakoid composition, biophysical techniques to determine alteration in light harvesting function and physiological experiments to analyse how processes at the whole plant level (efficiency, photoinhibition) have been altered.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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