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Gametic imprinting in Arabidopsis

ReferenceP08575
Principal Investigator / Supervisor Professor Roderick Scott
Co-Investigators /
Co-Supervisors
Institution University of Bath
DepartmentBiology and Biochemistry
Funding typeResearch
Value (£) 140,801
StatusCompleted
TypeResearch Grant
Start date 29/06/1998
End date 29/06/2001
Duration36 months

Abstract

Reciprocal crosses in Arabidopsis between diploid (2x) and autotetraploid (4x) parents reveal the operation of gametic imprinting within the endosperm. Deviations from the normal 2:1 ratio of maternal to paternal genomes in the endosperm reduce (maternal excess) or increase (paternal excess) the number of endosperm cells and the size of the mature seed. Maternal excess also slows the rate of embryogenesis. These parameters are easily measured and form the basis of an assay for the level of gametic imprinting. The assay will be used (i) to investigate the role of methylation in gametic imprinting by exploiting DNA hypomethylation mutants and the chemical demethylating agent, 5-azacytidine; and (ii) as the basis of a genetic screen for mutants affected in gametic imprinting.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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