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Functional analysis of genes encoding proteins expressed in the pollen coating of brassica oleracea
Reference
P08514
Principal Investigator / Supervisor
Professor Hugh Dickinson
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Plant Sciences
Funding type
Research
Value (£)
206,972
Status
Completed
Type
Research Grant
Start date
01/10/1997
End date
01/10/2000
Duration
36 months
Abstract
A combination of in vivo and in vitro bioassays has identified Brassica oleracea Pollen Coat Proteins (PCPs) as being active in both self- and interspecific incompatibility, and members of a sub-family (Class A) as interacting specifically with stigmatic S(incompatibility)-locus linked or related proteins. It is proposed to complete the cloning of all PCP-A genes encoding interacting proteins, to characterise their products fully, and analyse the nature of the interaction. The biological consequences of key interactions will be explored using reverse genetics, and a novel Arabidopsis/Brassica functional assay used to identify pollen- held interspecific and self-incompatibility determinants.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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