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Award details
Protein targeting and mobility in the plant secretory system
Reference
P08503
Principal Investigator / Supervisor
Professor Christopher Hawes
Co-Investigators /
Co-Supervisors
Institution
Oxford Brookes University
Department
Faculty of Health and Life Sciences
Funding type
Research
Value (£)
138,250
Status
Completed
Type
Research Grant
Start date
01/10/1998
End date
01/10/2001
Duration
36 months
Abstract
The potato virus X mediated expression system will be used to target green fluorescent protein (GFP) to the endomembrane system of tobacco leaf cells. Mobility of GFP tagged with KDEL, various calreticulin constructs and other vacuolar storage proteins will be assessed by recovery from photobleaching of brefeldin A induced sheets of endoplasmic reticulum. This will produce novel information on the mode of retention/retrieval of ER luminal proteins. This work will be coupled with the localisation of the GFP-tagged K/HDEL receptor to investigate recycling of KDEL proteins to and from the cis-Golgi. The ability of GFP-tagged mammalian Golgi transferases to partition into plant Golgi will be investigated and transgenic lines produced expressing fluorescent Golgi. These will be used in studies of Golgi dynamics in living plant cells.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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