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Functional characterisation of mutants of the PufX protein from Rhodobacter sphaeroides
Reference
P08459
Principal Investigator / Supervisor
Professor Mike Jones
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Division of Clinical Sciences (S)
Funding type
Research
Value (£)
38,706
Status
Completed
Type
Research Grant
Start date
01/09/1997
End date
01/09/1998
Duration
12 months
Abstract
The PufX protein is responsible for organising the proper assembly of the reaction centre/light harvesting complex 1 photosystem in Rhodobacter sphaeroides. Under funding from BBSRC grant 50/C01645, genetic engineering has been used to change the identity of the PufX protein, and to alter the primary sequence of the protein. Steady-state spectroscopy and growth studies have been used to determine the consequences of these changes for assembly of the photosystem. In the present study, the functional consequences of these changes will be examined by measuring the kinetics of light-driven cyclic electron transfer and membrane potential generation using a novel experimental system that consists of photosynthetic membranes that have been fused to liposomes.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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