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Genetic manipulation of the control of photosynthesis by SBPase

Reference	P08435
Principal Investigator / Supervisor	Professor Christine Raines
Co-Investigators / Co-Supervisors	Dr Julie Lloyd, Professor Stephen Long
Institution	University of Essex
Department	Biological Sciences
Funding type	Research
Value (£)	157,501
Status	Completed
Type	Research Grant
Start date	16/01/1998
End date	16/01/2001
Duration	36 months

Abstract

A fully active, deregulated form of the enzyme sedoheptulose-1,7-bisphosphatase (SBPase) will be introduced into both wild type and transgenic antisense SBPase tobacco plants. Transgenic plants will be selected on the basis of antibiotic resistance and the presence of the transgene confirmed using Southern analysis. The transgenic plants produced will be analysed for changes in the level and activity of SBPase protein by western blotting and enzyme assay techniques. Selected lines expressing the deregulated SBPase enzyme will be used to assess the impact that altered SBPase activity has on the flux of carbon through the Calvin cycle and on partioning of carbon to starch and sucrose. Non-invasive techniques will be used to assess photosynthetic performance of the transgenic SBPase tobacco plants grown in different light conditions. In addition the impact of light independent SBPase activity on carbon flux through the oxidative pentose phosphate pathway and into the shikimic acid pathway will be analysed in the transgenic plants.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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